Involvement of SDF-1 and MCP-1 in H2O2-induced ECM degradation in human dental pulp cells , Abstract Aim To determine whether chemokines such as SDF-1 and monocyte chemoattractant protein-1 (MCP-1) are responsible for H2O2-induced ECM degradation, and to identify the underlying mechanism in human dental pulp cells (HDPCs). Method HDPCS were exposed to 0.4 mM H2O2 for 48 h. mRNA and protein expression were examined by RT-PCR and Western blot analysis, respectively. The mRNA expression of chemokine (SDF-1 and MCP-1), their receptors (CXCR4 and CXCR2), extracellular matrix proteins was evaluated by reverse transcriptase-polymerase chain reaction. The production of SDF-1, MCP-1, CXCR4, and CCR2 in the culture medium was determined by enzyme-linked immunosorbent assay. Signal transduction pathway were examined by Western blotting. Results H2O2 provoked the activation of MCP-1 and SDF-1 mRNA and their respective receptors, CXCR4 and CXCR2. H2O2 treatment concomitantly down-regulated the expression of ECM molecules, such as type I collagen, elastin and fibronectin, and up-regulated the mRNA expression of matrix metalloproteinase-1 (MMP-1), MMP-2, MMP-8, and MMP-9. H2O2-induced ECM degradation and MMP up-regulation were blocked by neutralizing antibodies and siRNAs directed against SDF-1 and MCP-1. Inhibition of SDF-1 and MCP-1 blocked the H2O2-induced activation of Akt, p38, ERK, and NF-kB. Conclusion Inhibition of SDF and MCP-1 are a potent component of reducing ROS-induced ECM degradation in HDPCs, and may play an important role in pulpal and periapical inflammation. This article is protected by copyright. All rights reserved. , http://bit.ly/13gcao9

Involvement of SDF-1 and MCP-1 in H2O2-induced ECM degradation in human dental pulp cells , Abstract Aim To determine whether chemokines such as SDF-1 and monocyte chemoattractant protein-1 (MCP-1) are responsible for H2O2-induced ECM degradation, and to identify the underlying mechanism in human dental pulp cells (HDPCs). Method HDPCS were exposed to 0.4 mM H2O2 for 48 h. mRNA and protein expression were examined by RT-PCR and Western blot analysis, respectively. The mRNA expression of chemokine (SDF-1 and MCP-1), their receptors (CXCR4 and CXCR2), extracellular matrix proteins was evaluated by reverse transcriptase-polymerase chain reaction. The production of SDF-1, MCP-1, CXCR4, and CCR2 in the culture medium was determined by enzyme-linked immunosorbent assay. Signal transduction pathway were examined by Western blotting. Results H2O2 provoked the activation of MCP-1 and SDF-1 mRNA and their respective receptors, CXCR4 and CXCR2. H2O2 treatment concomitantly down-regulated the expression of ECM molecules, such as type I collagen, elastin and fibronectin, and up-regulated the mRNA expression of matrix metalloproteinase-1 (MMP-1), MMP-2, MMP-8, and MMP-9. H2O2-induced ECM degradation and MMP up-regulation were blocked by neutralizing antibodies and siRNAs directed against SDF-1 and MCP-1. Inhibition of SDF-1 and MCP-1 blocked the H2O2-induced activation of Akt, p38, ERK, and NF-kB. Conclusion Inhibition of SDF and MCP-1 are a potent component of reducing ROS-induced ECM degradation in HDPCs, and may play an important role in pulpal and periapical inflammation. This article is protected by copyright. All rights reserved. , http://bit.ly/13gcao9 , via Dental Teach " Daily Dental Info " http://www.facebook.com/photo.php?fbid=612177202140166&set=a.582976205060266.1073741849.110664842291407&type=1